Category: Hydrogen-ATPase

This genetic deletion is achieved through (i) heterologous recombination between the host chromosome and a suicide vector encoding a null allele and a resistance marker flanked by recombinase target sites, (ii) antibiotic-mediated selection for transformants, and (iii) subsequent excision of the resistance marker through transient expression of recombinase. risk can be minimized by increasing the… Continue reading This genetic deletion is achieved through (i) heterologous recombination between the host chromosome and a suicide vector encoding a null allele and a resistance marker flanked by recombinase target sites, (ii) antibiotic-mediated selection for transformants, and (iii) subsequent excision of the resistance marker through transient expression of recombinase

Twenty four hours later, DMEM with 25 g/mL puromycin (Sigma, St. Finally, knockdown Theobromine (3,7-Dimethylxanthine) of in cells resulted in FoxO3a-dependent gene expression patterns and increased radiosensitivity that partially mimicked those found in cells. Taken together, our data suggest a role for FoxO3a in the maintenance of genome integrity in response to DNA damage that… Continue reading Twenty four hours later, DMEM with 25 g/mL puromycin (Sigma, St