The cells were centrifuged for 10 minutes at 10,000 rpm at 4 C

The cells were centrifuged for 10 minutes at 10,000 rpm at 4 C. a hydrogel immobilized substrate for measuring the kinase activity of Met and Abl kinase from cancer cells. We immobilized kinase specific substrates into macroporous hydrogel micropillars in microchannels. These microchannels were incubated with 6 l of a kinase reaction solution containing cancer… Continue reading The cells were centrifuged for 10 minutes at 10,000 rpm at 4 C